Welcome to the Fluorosome Company. The Fluorosome Company develops and manufactures liposome-based fluorescence detection systems – Fluorosomes^® – for applications in drug discovery and related fields. Utilizing the Fluorosome^® platform we are continuously expanding our technology to develop new tools for “ADMET” studies in drug discovery.

The basic platform – Fluorosome^®-trans – measures passive drug diffusion through the membrane bilayer, yielding the property “membrane permeability”, which is predictive of tissue absorption e.g. oral absorption of a drug.

Our flagship product is the Fluorosome^®-trans-pgp assay. This high throughput inhibition assay is an innovative “stand alone” platform for characterizing P-glycoprotein/drug interactions. The P-glycoprotein is a key drug efflux pump responsible for poor oral drug absorption and penetration into the brain, as well as multi-drug resistance in tumors.  Unlike older vesicle assay systems formed from heterogeneous membrane fragments, Fluorosome-trans-pgp consists of highly purified functional P-glycoprotein in pure lipid vesicles.

Fluorosome^© -trans-pgp Inhibition Assay Advantages

• Specificity: Fluorosome®-trans-pgp contains only the P-glycoprotein transporter
• Low Sample Requirement: 50 μl or less of Fluorosome®-trans-pgp Reagent per assay means that only small amounts of test substances are required – e.g. a 10 μM drug inhibition assay requires ≤ 0.5 nanomole drug
• Speed: 1 minute per inhibition assay – e.g. an 8 point IC50 determination + 2 references takes only 10 minutes
• Cost: Cost is lower than conventional cell-based assays
• Convenience: Real time kinetic measurement and full analysis in a fluorescence microplate reader
• Popular format: 96 well half-volume or 384 well microplates – one well per control or inhibition assay
• Full coverage: inhibition at either or both the H and R sites of P-glycoprotein
• Dynamic Range: IC50 determinations over a concentration range of > 1000
• Simple assay procedure: No sterile conditions, non-compound specific, no radiolabel or LC-MS
• Amenable to robotics